A Facility Line STED super-resolution microscope from Abberior Instruments, built on an inverted Olympus IX83 body, with four excitation lasers (405, 488, 561, 640 nm) and a pulsed 775 nm STED laser. Adaptive-illumination methods (RESCue, DyMIN) allow live-cell imaging with substantially reduced photobleaching and phototoxicity, alongside FLIM capability, an Okolab stage-top incubator, and two single-photon-counting APD detectors.