3500 Genetic Analyzer for Resequencing & Fragment Analysis and Two RTqPCR instruments (ABI 7500 and 7500 Fast) are available for use at the Department of Biological Sciences. This facility enables researchers to perform highly sensitive, accurate, and reproducible quantitation of gene expression.

3500 Genetic Analyzer for Resequencing & Fragment Analysis

Identifying genetic variation, DNA sequencing through capillary electrophoresis, 3500 Series enables the highest levels of multiplexing for fragment analysis applications

▶ An 8 capillary system that can easily be upgraded to a 24-capillary system when you’re ready. Single-line, 505 nm, solid-state, long-life laser that utilizes a standard power supply and requires no heat-removal ducting, Powerful, integrated data collection and primary analysis software provides real-time assessment of data quality. Radio frequency identification (RFID) technology tracks key consumables data and records administrative information. Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes. Unrivaled application flexibility—one array and one polymer are used for most applications. Simple setup, operation and maintenance
▶ 3500 Series Data Collection software breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables and handy maintenance scheduling calendar functionality. New functionality includes simplified plate set-up and built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced on the instrument without the need to transfer output files into secondary analysis software packages.

7500 Fast & 7500 Real-Time PCR System

Applied Biosystems™ 7500 Real-Time PCR Systems offer you high performance, multicolor real-time PCR wherever your research takes you.

▶ The Applied Biosystems 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™/SYBR® Green I, VIC®/JOE™, NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™ dyes
▶ TaqMan® Fast Universal PCR Master Mix and Fast SYBR® Green Master Mix enable-rapid results with excellent assay performance equivalent to that of standard real-time PCR
▶ The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time for labs running a variety of applications, including High Resolution Melting (HRM) analysis.

CFX96 Touch Real-Time PCR Detection System

▶ The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.
▶ Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.
CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels: The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

Email at sequencing@iiserkol.ac.in for Booking your slot

Faculty in-charge: Dr. Amirul Islam Mallick (email: amallick@iiserkol.ac.in)

Technician: Mr. Somnath Halder (email: som.biochem@iiserkol.ac.in)